Review



anti mouse nk1 1 pk 136 pe cy7  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Thermo Fisher anti mouse nk1 1 pk 136 pe cy7
    Study of ROS levels in NKT and CD4 T cells. (A) Freshly isolated thymocytes, splenocytes and liver lymphocytes were compared for the amount of ROS using 2’,7’-dichlorofluorescin diacetate (DCFDA) as described in Materials and Methods. NKT cells were identified by TCR-β low and CD1d-tetramer+. N= 8 mice per group. (B) Expression level of CD44, CD62L, and <t>NK1.1</t> was used to compare the amount of ROS in two subsets of splenic NKT cells. N=4 mice per group. (C) NKT, CD44+ and CD44− CD4 T cells, and NK cells from spleen and liver were compared for the levels of ROS. N=6 mice per group. (D) NKT and CD4 T cells from total splenocytes were compared for mitochondria-produced ROS using MitoSox, as described in the Methods. N=3 mice per group. (E) Gene expression of the members of the Nox gene family was assessed from sorted splenic NKT and CD4 T cells using qPCR. Gene expression was normalized to either β-actin or GAPDH. N=4 mice per group. All flow cytometric data were analyzed with live cells and C57BL/6 mice were used. One representative histogram is shown from at least three independent experiments. Error bars represent the mean ± SEM. **p <0.01; ***p<0.001; ****p<0.0001.
    Anti Mouse Nk1 1 Pk 136 Pe Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse nk1 1 pk 136 pe cy7/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    anti mouse nk1 1 pk 136 pe cy7 - by Bioz Stars, 2026-04
    86/100 stars

    Images

    1) Product Images from "Reactive Oxygen Species Regulate the Inflammatory Function of NKT Cells through Promyelocytic Leukemia Zinc Finger"

    Article Title: Reactive Oxygen Species Regulate the Inflammatory Function of NKT Cells through Promyelocytic Leukemia Zinc Finger

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700567

    Study of ROS levels in NKT and CD4 T cells. (A) Freshly isolated thymocytes, splenocytes and liver lymphocytes were compared for the amount of ROS using 2’,7’-dichlorofluorescin diacetate (DCFDA) as described in Materials and Methods. NKT cells were identified by TCR-β low and CD1d-tetramer+. N= 8 mice per group. (B) Expression level of CD44, CD62L, and NK1.1 was used to compare the amount of ROS in two subsets of splenic NKT cells. N=4 mice per group. (C) NKT, CD44+ and CD44− CD4 T cells, and NK cells from spleen and liver were compared for the levels of ROS. N=6 mice per group. (D) NKT and CD4 T cells from total splenocytes were compared for mitochondria-produced ROS using MitoSox, as described in the Methods. N=3 mice per group. (E) Gene expression of the members of the Nox gene family was assessed from sorted splenic NKT and CD4 T cells using qPCR. Gene expression was normalized to either β-actin or GAPDH. N=4 mice per group. All flow cytometric data were analyzed with live cells and C57BL/6 mice were used. One representative histogram is shown from at least three independent experiments. Error bars represent the mean ± SEM. **p <0.01; ***p<0.001; ****p<0.0001.
    Figure Legend Snippet: Study of ROS levels in NKT and CD4 T cells. (A) Freshly isolated thymocytes, splenocytes and liver lymphocytes were compared for the amount of ROS using 2’,7’-dichlorofluorescin diacetate (DCFDA) as described in Materials and Methods. NKT cells were identified by TCR-β low and CD1d-tetramer+. N= 8 mice per group. (B) Expression level of CD44, CD62L, and NK1.1 was used to compare the amount of ROS in two subsets of splenic NKT cells. N=4 mice per group. (C) NKT, CD44+ and CD44− CD4 T cells, and NK cells from spleen and liver were compared for the levels of ROS. N=6 mice per group. (D) NKT and CD4 T cells from total splenocytes were compared for mitochondria-produced ROS using MitoSox, as described in the Methods. N=3 mice per group. (E) Gene expression of the members of the Nox gene family was assessed from sorted splenic NKT and CD4 T cells using qPCR. Gene expression was normalized to either β-actin or GAPDH. N=4 mice per group. All flow cytometric data were analyzed with live cells and C57BL/6 mice were used. One representative histogram is shown from at least three independent experiments. Error bars represent the mean ± SEM. **p <0.01; ***p<0.001; ****p<0.0001.

    Techniques Used: Isolation, Expressing, Produced



    Similar Products

    anti mouse nk1 1 pk 136 pe cy7  (Thermo Fisher)
    86
    Thermo Fisher anti mouse nk1 1 pk 136 pe cy7
    Study of ROS levels in NKT and CD4 T cells. (A) Freshly isolated thymocytes, splenocytes and liver lymphocytes were compared for the amount of ROS using 2’,7’-dichlorofluorescin diacetate (DCFDA) as described in Materials and Methods. NKT cells were identified by TCR-β low and CD1d-tetramer+. N= 8 mice per group. (B) Expression level of CD44, CD62L, and <t>NK1.1</t> was used to compare the amount of ROS in two subsets of splenic NKT cells. N=4 mice per group. (C) NKT, CD44+ and CD44− CD4 T cells, and NK cells from spleen and liver were compared for the levels of ROS. N=6 mice per group. (D) NKT and CD4 T cells from total splenocytes were compared for mitochondria-produced ROS using MitoSox, as described in the Methods. N=3 mice per group. (E) Gene expression of the members of the Nox gene family was assessed from sorted splenic NKT and CD4 T cells using qPCR. Gene expression was normalized to either β-actin or GAPDH. N=4 mice per group. All flow cytometric data were analyzed with live cells and C57BL/6 mice were used. One representative histogram is shown from at least three independent experiments. Error bars represent the mean ± SEM. **p <0.01; ***p<0.001; ****p<0.0001.
    Anti Mouse Nk1 1 Pk 136 Pe Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse nk1 1 pk 136 pe cy7/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    anti mouse nk1 1 pk 136 pe cy7 - by Bioz Stars, 2026-04
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Study of ROS levels in NKT and CD4 T cells. (A) Freshly isolated thymocytes, splenocytes and liver lymphocytes were compared for the amount of ROS using 2’,7’-dichlorofluorescin diacetate (DCFDA) as described in Materials and Methods. NKT cells were identified by TCR-β low and CD1d-tetramer+. N= 8 mice per group. (B) Expression level of CD44, CD62L, and NK1.1 was used to compare the amount of ROS in two subsets of splenic NKT cells. N=4 mice per group. (C) NKT, CD44+ and CD44− CD4 T cells, and NK cells from spleen and liver were compared for the levels of ROS. N=6 mice per group. (D) NKT and CD4 T cells from total splenocytes were compared for mitochondria-produced ROS using MitoSox, as described in the Methods. N=3 mice per group. (E) Gene expression of the members of the Nox gene family was assessed from sorted splenic NKT and CD4 T cells using qPCR. Gene expression was normalized to either β-actin or GAPDH. N=4 mice per group. All flow cytometric data were analyzed with live cells and C57BL/6 mice were used. One representative histogram is shown from at least three independent experiments. Error bars represent the mean ± SEM. **p <0.01; ***p<0.001; ****p<0.0001.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Reactive Oxygen Species Regulate the Inflammatory Function of NKT Cells through Promyelocytic Leukemia Zinc Finger

    doi: 10.4049/jimmunol.1700567

    Figure Lengend Snippet: Study of ROS levels in NKT and CD4 T cells. (A) Freshly isolated thymocytes, splenocytes and liver lymphocytes were compared for the amount of ROS using 2’,7’-dichlorofluorescin diacetate (DCFDA) as described in Materials and Methods. NKT cells were identified by TCR-β low and CD1d-tetramer+. N= 8 mice per group. (B) Expression level of CD44, CD62L, and NK1.1 was used to compare the amount of ROS in two subsets of splenic NKT cells. N=4 mice per group. (C) NKT, CD44+ and CD44− CD4 T cells, and NK cells from spleen and liver were compared for the levels of ROS. N=6 mice per group. (D) NKT and CD4 T cells from total splenocytes were compared for mitochondria-produced ROS using MitoSox, as described in the Methods. N=3 mice per group. (E) Gene expression of the members of the Nox gene family was assessed from sorted splenic NKT and CD4 T cells using qPCR. Gene expression was normalized to either β-actin or GAPDH. N=4 mice per group. All flow cytometric data were analyzed with live cells and C57BL/6 mice were used. One representative histogram is shown from at least three independent experiments. Error bars represent the mean ± SEM. **p <0.01; ***p<0.001; ****p<0.0001.

    Article Snippet: Flow cytometry assay The following antibodies were used: anti-mouse TCR-β (H57-597) APC or Pacific Blue, PBS57 loaded CD1d tetramer APC or Pacific Blue, anti-mouse CD4 (GK1.5) PerCp-Cy5.5, anti-mouse CD8 (53-6.7) Am-cyan, anti-mouse NK1.1 (PK-136) PE-Cy7, anti-mouse CD44 (IM7) PerCp-Cy5.5, anti-mouse CD69 (H1-2F3) PE-Cy7, anti-mouse CD62L (MEL-14) eVolve605, anti-mouse IFN-γ (XMG1.2) FITC or APC, anti-mouse IL-4 (11B11) PE-Cy7, anti-mouse IL-17 (TC11-18H10) APC-eFluor780, anti-T-bet (eBio4B10) FITC, anti-RORγt (AFKJS-9) Pacific Blue, anti-GATA3 (L50-823) APC, and anti-PLZF (Mags-21F7) PE (all from eBioscience).

    Techniques: Isolation, Expressing, Produced